Fig. 1
Schematic of TsTGH2 protein and multiple sequence alignment of TsTGH2 with homologs. (A) Domain structure of TsTGH2 consists of signal peptide (amino acid residues (aa) 1−23), pro-TsTGH2 (pTsTGH2) (aa24–423) containing proteolytic cleavage site motif, RPKR, and mature-TsTGH2 (mTsTGH2) (aa272–423) with nine cysteines (C) inside domain. The arrow indicates the furin cleavage site. (B) The deduced amino acid sequence of mTsTGH2 was aligned with TGF-β superfamily members from other representatives using MUSCLE. The conserved cysteine residues among TGF-β superfamily are shaded in red and additional conserved residues (tryptophan [W], proline [P], and glycine [G]) are shaded in blue. The stars indicate two of conserved cysteines found only in TGF-β/activin/inactivin subfamily. Gaps are represented with a dash (-). Ident/Sim (%) represents percent identity and similarity between TsTGH2 and each representative sequence. The GenBank accession numbers of the sequence are provided in Supplementary Information File 1: Table S1.
