Fig. 4 | Scientific Reports

Fig. 4

From: Molecular and biological characterization of transforming growth factor-β homolog derived from Trichinella spiralis

Fig. 4

Expression of rTsTGH2s in prokaryotic expression system. (A) recombinant pro-TsTGH2 (rpTsTGH2) and (B) recombinant mature-TsTGH2 (rmTsTGH2) were successfully expressed in E. coli. Lane M: PageRuler Prestained Protein Ladder (Thermo Fisher Scientific Inc.); Lane 1: non-induction; Lane 2: IPTG induction; Lane 3: insoluble fraction; Lane 4: soluble fraction; Lane 5: purified rTsTGH2; Lane 6: western blot analysis of rTsTGH2 probe with mouse anti-His tag antibody. Arrowhead and arrow indicate rpTsTGH2 and rmTsTGH2, respectively.

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