Fig. 8 | Scientific Reports

Fig. 8

From: Molecular and biological characterization of transforming growth factor-β homolog derived from Trichinella spiralis

Fig. 8

Expression of recombinant TsTGH2 (rTsTGH2) in mammalian expression system. HEK293T cells were transfected with pSecTag2A carrying pro-TsTGH2 (pSecTag2A/pTsTGH2), pSecTag2A carrying mature-TsTGH-2 (pSecTag2A/mTsTGH2) or pSecTag2A carrying human prostate specific antigen (pSecTag2A/PSA). The rTsTGH2 expression was detected in the cell lysates (A) and the culture media (B) using western blot probed with mouse anti-His tag antibody. Lane M: PageRuler Prestained Protein Ladder (Thermo Fisher Scientific Inc.); Lane 1: only polyethylenimine [PEI] (Mock transfected); Lane 2: transfected empty pSecTag2A vector (negative control); Lane 3: transfected with pSecTag2A/PSA (positive control); Lane 4: transfected with pSecTag2A/mTsTGH2 and Lane 5: transfected with pSecTag2A/pTsTGH2.

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