Fig. 9
Effect of recombinant pro-TsTGH2 (rpTsTGH2) on activation of SBE luciferase reporter stable HEK293 cell line. Cells were treated with different concentrations of pTsTGH2 or human TGFβ (hTGFβ). Untreated SBE Reporter-HEK293 cells were used as the negative control. Recombinant human PSA (rhPSA) at 10 µg/mL (rhPSA), serving as an irrelevant control, did not activate luciferase activity. The luciferase activities were measured using a luminometer. Statistical analysis was performed using an unpaired t-test. *p = 0.0399; **p = 0.0028; ***p = 0.0031. Data are represented as mean ± SD. The experiments were performed in triplicate and repeated three times.
