Fig. 1

Schematic representation of the experimental design. (A) In Experiment 1, female mice were daily treated with the vehicle or Ganirelix^® at the indicated doses from postnatal day 10 (PND10) to PND16. (B) In Experiment 2, female mice were daily treated with the vehicle, flutamide (F), letrozole (L) or both (F/L) at the indicated doses from PND10 to PND17. For both experiments, body weight, pubertal onset and estrous cyclicity were monitored in intact females. Females were then ovariectomized and received estradiol implants (OVX + E2) and were primed with progesterone (Pg) before each behavioral test. At the end of behavioral tests, females were euthanized and tissues were collected.