Fig. 4

Effects of separate or combined treatment with flutamide and letrozole during minipuberty on pubertal onset, estrous cyclicity and body growth. A-C. Female mice treated with the vehicle (Veh), flutamide (F), letrozole (L) or both (F/L) from postnatal day (PND)10 to PND17 were analyzed for the age at vaginal opening (A), age at first estrus (B) and interval between the two pubertal markers (C). *p < 0.05 for the indicated groups. D-E. Duration of the estrous cycle (D) and of each stage (E) in adult females. F. Body weight was monitored weekly from PND21 to PND86, ^ap <0.05 between Veh and L, ^bp < 0.05 between Veh and F/L, ^cp < 0.05 between F and L). Data are presented as means ± S.E.M. (n = 8–13 per treatment).