Fig. 5

Effects of separate and combined treatment with flutamide and letrozole during minipuberty on female sexual behavior. A-B. In the olfactory preference test, female mice treated with the vehicle (Veh), flutamide (F), letrozole (L) or both (F/L) had choice to chemoinvestigate anesthetized male or female stimuli (A). The discrimination index, time spent by exposed females in male investigation minus the time spent in female investigation divided by the total time of investigation (B). *p < 0.05, positive index for the four groups. C-E. Lordosis behavior was tested for sexually-experienced females in the presence of sexually experienced males for 30 min (C). The number of male mounts (D) and lordosis quotient (E) are indicated. F-G. In the three-chamber test, sexually experienced males had choice to investigate either Veh- or F-, Veh- or L-, or Veh- or F/L-treated females (**p < 0.01 for F/L) (F). The percentage of time spent investigating each female is indicated (G). H-I. In the urinary preference test, sexually experienced males had choice to investigate urine either from Veh- or F-, Veh- or L-, or Veh- or F/L-treated females (H). The percentage of time spent investigating each urine stimulus is indicated (I). Data are presented as means ± S.E.M. (n = 10–13 per treatment).