Fig. 3

Differential abundance of prokaryotes and fungi in zaxinone and MiZax treatments compared to the control (acetone). (A-C) Number of enriched and depleted ASVs in the three compartments considered (endosphere, rhizosphere, unplanted soil) at tillering (T1) and milky-stage maturation (T2) in prokaryotes (A) and fungi (C). (B-D) Circular phylogenetic tree and heatmap of the bacterial (C) and fungal (D) ASVs with higher or lower microbial loads in the endosphere of zaxinone/MiZax treated plants compared with acetone-treated controls. Only ASV with a relative abundance higher than 5% and classified at least at Family rank are displayed. Maximum likelihood (ML) phylogenetic trees were constructed using differentially abundant ASV sequences and tree branches were coloured according to taxonomy (sensu UNITE v8) at the phylum level. The heatmaps shows log₂fold-change values indicating ASVs enriched (red) and depleted (blue) in treatments compared with the acetone control. Stars represent ASVs consistently enriched or depleted compared to the control in at least two conditions.