Fig. 1

Annotation of our de novo R. atratulus mitogenome assembly using MitoFish, visualized with Chloroplot (Zheng et al., 2020). The innermost ring represents the GC content of a given 100 bp interval of the sequence, with the subdivided rings representing increasing 25% intervals. The arrows in this section correspond to the direction of transcription for the genes and RNAs displayed in the outer ring, with the inner features transcribed clockwise (forward strand) and the outer features transcribed counterclockwise (reverse strand). Features are color coded by their product, per the key. The inset photograph is of a male R. atratulus individual in breeding colors. This individual was collected and photographed by the authors from the same locality as the sequenced individual in May 2023.