Fig. 1

Illustration of conventional and HistoBrick embedding. (a) Photograph of a conventionally embedded block (left) and schematic illustration of a block with conventional embedding of multiple retinal organoids from one experimental condition (middle). Resulting organoid distribution in the different slides prepared during cryosectioning (right). (b) Photograph of a HistoBrick with arrowheads pointing to individual organoids. Some organoids are completely white, while some organoids contain pigmented retinal pigment epithelium cells appearing black on the photograph (left, scale bar: 5 mm). Schematic illustration of a HistoBrick filled with 16 retinal organoids of different experimental conditions (middle). Resulting organization of the slides obtained after cryosectioning (right).