Fig. 2
Schematic of HistoBrick cryoblock fabrication steps. 1. The silicone mold is filled with the liquid hydrogel solution; 2. The hydrogel solution is crosslinked either by UV or at 4 °C for PEGDA and gelatine respectively; 3. The gel well plate is unmolded; 4. The retinal organoids are incubated in the embedding matrix (same hydrogel solution as for the fabrication of the gel well plate) at 37 °C; 5. The organoids are transferred with the embedding matrix into the gel well plate, sedimented to the bottom of the plate, followed by crosslinking; 6. Snap freezing. Photograph of the silicone mold and gel well plate (bottom left, scale bar: 10 mm). HistoBrick containing the gel well plate, organoids and embedding matrix (bottom right, scale bar: 1 mm).
