Fig. 3
Comparison of different embedding materials on cryosectioning and rheological properties. (a) Brightfield images of the HistoBrick cryosections (of 20 µm thickness) made of PEGDA (8%), gelatine (7.5%) and PEGDA-gelatine (8% PEGDA and 2.5% gelatine) solution, showing the interface (arrowheads) between the HistoBrick well plate and the embedding matrix. No microtissues were embedded in the HistoBricks and thus no organoids are present in the sections. (scale bar: 1 mm). (b) H&E staining of retinal organoids (all organoids were fixed in week 33, n = 4 organoids for each embedding) showing the interface of the embedding matrix and organoids. For gelatine HistoBrick embedding, PEGDA-gelatine HistoBrick embedding, and OCT conventional embedding, sections are of 20 µm thickness (scale bar: 100 μm). (c) Complex viscosity in function of time with a temperature profile going from 37 to 20 °C and stabilizing.
