Fig. 5
Enhanced throughput and traceability with HistoBrick. Consecutive cryosections (20 µm sectioning thickness) of HistoBrick showing 16 organoids. All organoids are 33 weeks old. Sections stained with (a) Hoechst (scale bar: 1 mm) and (b) H&E (scale bar: 5 mm). (c) Zoom of one retinal organoid from section n°23, highlighted with red dashed line in (a), and n°25, highlighted with blue dashed line in (b), showing the correlation between Hoechst and H&E staining (scale bars: 100 µm). (d) Consecutive cryosections of one H&E stained organoid from section n°25, highlighted with green dashed line in (b), revealing some aspects of retinal organoid 3D organization (scale bars: 100 µm).
