Fig. 2
Time-dependent effects of structurally different arsenicals on cutaneous injury in Ptch1+/-/SKH1 hairless mice model. (A) The experimental design depicts the timeline of cutaneous arsenical exposures, clinical observations and skin sample collection. (B) Representative images showing gross skin injury effects with time following DPCA, DPCYA, or DECA exposure in mice. (C) Clinical scores, including measurements of skin bifold thickness and Draize score, were documented after 6 h, 24 h, and 72 h of arsenical exposure. (D) H&E staining of skin sections from the control and arsenicals exposed mice showing the infiltration of inflammatory cells (green arrows) and complete loss of epidermal cells (red arrows). Epi- Epidermis, Der-Dermis, Scale bar- 50 µm. (E) Histograms showing the average number of micro-vesicants/unit area of the skin sections. epidermal-dermal separation was recorded to count micro-vesication/slide sections at 200 × magnification. *P < 0.05, ***P < 0.001, ****P < 0.0001 showing significance compared to vehicle treated controls. ns-non-significant. N = 4–6/group.
