Fig. 1
From: A single vector system for tunable and homogeneous dual gene expression in Escherichia coli
Population-homogeneous gene expression using IPTG and arabinose. (A) Top panel shows the percentage of IPTG-induced sfGFP expression measured using flow cytometry. Middle panel shows florescence intensity as a function of inducer concentration for expression of sfGFP in Tuner[DE3] cells measured in plates (black symbols) or by flow cytometry (white symbols, averages of three colonies, error bars indicate standard error of measurement). Bottom panel shows flow cytometry population distribution curves as a function of IPTG concentration for colony 1. (B) Percentage of arabinose-induced cells (top panel) and sfGFP fluorescence intensity (middle panel) as a function of arabinose concentration. Cells were either measured directly in plates (black symbols) or using flow cytometry (white symbols). Bottom panel shows the corresponding population distribution curves as a function of arabinose concentration for colony 1.
