Fig. 12
Mechanistic study of NET1 in TNBC cells. (A) BP and (B) KEGG enrichment analyses of DEGs in NET1-overexpressing cells compared with non-NET1-overexpressing MDA-MB-231 cells. (E) Changes in the protein levels of AKT and P-AKT (Ser473) in TNBC cell lines with and without NET1 overexpression. (D) Semiquantitative statistical analysis of the changes in AKT and P-AKT (Ser473) protein levels in TNBC cell lines with and without NET1 overexpression. (E) Changes in the protein levels of NET1 and P-AKT (Ser473) after treatment with a P-AKT inhibitor (MK2206) in SUM-159PT cells overexpressing NET1. (F) Semiquantitative statistical analysis of the changes in the protein levels of NET1 and P-AKT (Ser473) after treatment with a P-AKT inhibitor (MK2206). (G-I) CCK-8 assay of changes in the proliferation of TNBC cells with and without NET1 overexpression after treatment with a P-AKT inhibitor (MK2206). Abbreviations: BP, biological process; KEGG, Kyoto Encyclopedia of Genes and Genomes; ns, not significant, **P < 0.01, ***P < 0.001, and ****P < 0.0001.
