Fig. 5
Social behaviors of the Frrs1l+/− mice. (A–E) Results of the sociability tests. (A) Representative heat map images. (B) Both the wildtype and Frrs1l+/− mice preferred interaction with a conspecific mouse to the empty wire cup (n = 16, WT; n = 17, Frrs1l+/−; stimulus × genotype F (1,31) = 0.0178, P = 0.8949; stimulus F (1,31) = 57.11, P < 0.0001; genotype F (1,31) = 1.236, P = 0.2749; Two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test). (C) There was no significant difference in the discrimination index (t (31) = 0.3550, P = 0.725, unpaired two-tailed t-test). (D) There was no significant difference in the total interaction time, defined as the sum of the durations during which the subject mice interacted with the conspecific mouse and the empty cup (t (31) = 1.112, P = 0.2749, unpaired two-tailed t-test). (E) There was no significant difference in time spent in center chamber during sociability tests (t (31) = 0.4247, P = 0.6740, unpaired two-tailed t-test). (F) Results of number of interactions in the sociability tests (stimulus × genotype F (1,31) = 0.0502, P = 0.8243; stimulus F (1,31) = 57.82, P < 0.0001; genotype F (1,31) = 0.0257, P = 0.8736; Two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test). (G) Results of mean duration of single interaction time (mean duration of time spent during one bout of interaction) in the sociability tests (stimulus × genotype F (1,31) = 0.0072, P = 0.9329; stimulus F (1,31) = 7.961, P = 0.0083; genotype F (1,31) = 3.547, P = 0.0691; Two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test). (H–N) Results of the tests for social novelty recognition tests. (H) Representative heat map images. (I) Whereas the wildtype mice spent more time interacting with a novel mouse than with a familiar mouse, the Frrs1l+/− mice showed no significant difference in preference to the familiar and novel mouse, with a trend toward slightly increased preference to the familiar mouse compared to WT mice (stimulus × genotype F (1,31) = 12.83, P = 0.0012; stimulus F (1,31) = 2.223, P = 0.1461; genotype F (1,31) = 0.2662, P = 0.6095; Two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test). (J) Discrimination index of the Frrs1l+/− mice was significantly lower than that of the wildtype mice (t (31) = 3.733, P = 0.0008, unpaired two-tailed t-test). (K) There was no significant difference in the total interaction time, defined as the sum of the durations during which the subject mice interacted with the familiar mouse and the novel mouse (t (31) = 0.5160, P = 0.6095, unpaired two-tailed t-test). (L) There was no significant difference in time spent in center chamber during social novelty recognition tests (t (31) = 0.3744, P = 0.7107, unpaired two-tailed t-test). (M) Results of number of interactions in the social novelty recognition tests (stimulus × genotype F (1,31) = 15.14, P = 0.0005; stimulus F (1,31) = 0.5754, P = 0.4538; genotype F (1,31) = 0.9568, P = 0.3356; Two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test). (N) Results of mean duration of single interaction time in the social novelty recognition tests (stimulus × genotype F (1,31) = 0.2341, P = 0.6319; stimulus F (1,31) = 1.398, P = 0.2461; genotype F (1,31) = 0.0029, P = 0.9572; Two-way repeated measures ANOVA with Bonferroni’s multiple comparisons test). Data are presented as mean \(\pm\) s.e.m. ns, not significant. *P < 0.05, **P < 0.01, ****P < 0.0001.
