Fig. 2
From: Role of P2 X7 receptor during focused ultrasound induced blood brain barrier modulation
Regulation of P2 X7 receptors and downstream signaling pathways after sonication. (a) Representative IHC images of DAPI (blue), P2 X7 receptor (white arrow, green) and RECA-1 (white arrow, red) in the hippocampal region of the brain after LiFUS. Scale bar: 100 μm. (b,c) Western blotting and RT-PCR results for P2 X7 receptors. The bar graph shows the normalized level of the receptors relative to the control. Band intensity images for each group are also included. * p < 0.05, ** p < 0.01, *** p < 0.001; compared to 1 h, # p < 0.05, ### p < 0.001; compared to Con. (d,e) Western blot and RT-PCR results for NLRP3 inflammasome. The analysis method employed for NLRP3 was the same as that for the P2 X7 receptors. ** p < 0.01, *** p < 0.001; compared to 1 h, & p < 0.05, && p < 0.01; compared to 4 h. (f,g) Western blot and RT-PCR results for IL-1ß. The analysis method employed for NLRP3 was the same as that for the P2 × 7 receptors. * p < 0.05, ** p < 0.01, *** p < 0.001; compared to 1 h, # p < 0.05; compared Con. All data are represented as mean ± SD (n = 5). One-way ANOVA with Tukey’s multiple comparison test was performed.
