Fig. 1

The effect of serotonin and dopamine on proximity labelling. (A) Representative images showing the effect of increasing serotonin concentrations on HRP-mediated biotinylation in HEK293T cells at varying BxxP concentrations. (B) Quantitative analysis of serotonin’s effect on HRP-mediated biotinylation at BxxP concentrations of (i) 10 µM, (ii) 50 µM, and (iii) 100 µM. Data are presented as the mean of n = 5 random views per serotonin concentration from three independent experiments. (C) Representative images showing the effect of increasing serotonin concentrations on APEX2-mediated biotinylation in HEK293T cells at varying BxxP concentrations. (D) Quantitative analysis of serotonin’s effect on APEX2-mediated biotinylation at BxxP concentrations of (i) 10 µM, (ii) 50 µM, and (iii) 100 µM. Data are presented as the mean of n = 5 random views per serotonin concentration from three independent experiments. (E) Representative images showing the effect of increasing dopamine concentrations on HRP-mediated biotinylation in HEK293T cells at varying BxxP concentrations. (F) Quantitative analysis of dopamine’s effect on HRP-mediated biotinylation at BxxP concentrations of (i) 10 µM, (ii) 50 µM, and (iii) 100 µM. Data are presented as the mean of n = 5 random views per serotonin concentration from three independent experiments. Scale bars represent 100 µm. Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test, *, **, ***, **** represent p ≤ 0.05, 0.01, 0.001 and 0.0001 respectively. ns: non-significant.