Fig. 3

The effect of serotonin on the biotinylation reaction radius in HRP-expressing primary neurons, with and without Dz-PEG preincubation. (A) Representative images showing the effect of 10 µM serotonin alone and 10 µM serotonin with 500 µM Dz-PEG preincubation on biotinylation level in primary cortical neurons expressing HRP on the membrane. 8-bit pseudo-colour images represent the threshold used when quantifying the fluorescence intensity and area of HRP and NA for the result in B. (B) Quantification of the effects of 10 µM serotonin alone and 10 µM serotonin with 500 µM Dz-PEG preincubation on biotinylation level depicted by (i) fluorescence intensity of NA normalised by HRP expression, (ii) labelling area of NA normalised by HRP expression. Scale bars represent 100 µm. n = 5 neurons in each condition from one experiment. Data are presented as mean ± SD. One-way ANOVA with Dunnett’s multiple comparisons test, *, **, ***, **** represent p ≤ 0.05, 0.01, 0.001 and 0.0001 respectively.