Fig. 1 | Scientific Reports

Fig. 1

From: Global proteomics and affinity mass spectrometry analysis of human Schwann cells indicates that variation in and loss of neurofibromin (NF1) alters protein expression and cellular and mitochondrial metabolism

Fig. 1

PPI interactions between tagged mNf1 in ipn97.4 cells compared to tagged mNf1 in HEK293 cells. (A) Venn diagram of proteins specific to HEK293 transiently transfected with mNf1 cDNA or ipn97.4 NF1 -/- cells stably transfected with mNf1 cDNA. B) NF1 PPI interactions seen in HEK293 NF1 -/- cells transfected with WT tagged Nf1 and in ipnIP97.4 NF1 -/- cells stably transfected with WT tagged Nf1 or HEK293 empty vector (EV) control cells. Average for each group is based on normalized spectral counts. C) Venn diagram of proteins specific to either WT or variant cDNA after nonspecific PPI’s were filtered out. (D) All protein interactions with NF1 that were significantly altered between the specified variant and WT NF1.

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