Fig. 4 | Scientific Reports

Fig. 4

From: Global proteomics and affinity mass spectrometry analysis of human Schwann cells indicates that variation in and loss of neurofibromin (NF1) alters protein expression and cellular and mitochondrial metabolism

Fig. 4

Western blot validation of selected proteins from IPA pathways of interest. (A) Representative Western blot of selected proteins and (B) Quantification of multiple independent western blots (N > 3). (LAMC1 and CYB5R3: WT-9 n = 4; delM992-12 n = 5; R1809C-4 n = 5; G848R-3 n = 4; R1276Q-1 n = 5); (SOD2, CAPN2, and CBP: WT-9 n = 4; delM992-12 n = 4; R1809C-4 n = 3; G848R-3 n = 4 R1276Q-1 n = 4); (PFK1: WT-9 n = 5; delM992-12 n = 5; R1809C-4 n = 6; G848R-3 n = 4; R1276Q-1 n = 5); (UQCRC2: WT-9 n = 3; delM992-12 n = 4; R1809C-4 n = 3; G848R-3; n = 4 R1276Q-1 n = 4); (NDUFS3: WT-9 n = 4; delM992-12 n = 5; R1809C-4 n = 4; G848R-3 n = 3; R1276Q-1 n = 5) (* p-value > 0.05; ** p-value > 0.01 compared to WT-9; measured via a one-way T-test with equal variance after normalization of the data). Most cell lines had multiple clones created; we chose a single clone for each cell line for Western blot validation via expression of NF1 (Supplemental Fig. 2A).

Back to article page