Fig. 1
Overview of the effect of lipidation position and moiety on potency of lipidated hαCGRP8-37 analogues. Six different lipidation moieties were applied to a lysine substitution at every amino acid residue of hαCGRP8-37. A γGlu linker was incorporated between the peptide and the connected C18DA or C20DA. The peptides were incubated with a fixed concentration of hαCGRP8-37 corresponding to its EC90 (24 pM). Five-point concentration response curves were generated with antagonist concentrations of 30 nM, 100 nM, 300 nM, 1 µM and 3 µM. Antagonist potency was estimated as pIC50, which refer to the logarithmic concentration of antagonist required to induce 50% inhibition of the cAMP levels generated from the fixed concentration of hαCGRP8-37. Datapoints represent technical replicates from a single independent experiment (n = 1).
