Fig. 2
From: A multicenter study on TROP2 as a potential targeted therapy for extramammary Paget disease in Japan
Effect of TROP2 knockdown on proliferation of KS-EMPD-1 cells. TROP2 was inhibited by siRNA and the effect of this on cell proliferation was investigated. (A) Knockdown efficiency of TROP2 mRNA. Mean ± SD of relative TROP2 expression calculated from three independent experiments is shown. *p < 0.05. (B) Knockdown efficiency of TROP2 protein. Representative blot images and mean ± SD of TROP2 expression calculated from three independent experiments are shown. Original, uncropped blot images are shown in Supplementary Fig. S1. *p < 0.05 and **p < 0.01. (C) TROP2 was inhibited by siRNA and viable cells were quantified using CCK-8 solution. Experiments were performed in five wells for each condition and were independently repeated three times. Absorbance at 450 nm which reflects the number of viable cells is shown. *p < 0.05 and ***p < 0.001. (D) Expression of cell proliferation-related genes (CCND1 and C-MYC), survival-related genes (MCL1, BCL2, and BCL-XL), and apoptosis-related genes (BAX and BAK1) in negative control or TROP2 siRNA-transfected cells. Mean ± SD of mRNA expression calculated from three independent experiments is shown. *p < 0.05, **p < 0.01, and ***p < 0.001.
