Fig. 3
From: A multicenter study on TROP2 as a potential targeted therapy for extramammary Paget disease in Japan
Effect of TROP2 knockdown on migration of KS-EMPD-1 cells. TROP2 was inhibited by siRNA and the effect of this on cell migration was investigated. (A) Gene expression of matrix metalloproteases (MMP1, 2, 3, 9, 10, 12, 13, and 14) in negative control or TROP2 siRNA-transfected cells. Mean ± SD of mRNA expression calculated from three independent experiments is shown. ***p < 0.05, **p < 0.01, and ***p < 0.001. (B) Expression of cell migration-related genes (CDH1, VIM, SNAI1, TWIST1, ZEB1, and ZEB2) in negative control or TROP2 siRNA-transfected cells. Mean ± SD of mRNA expression calculated from three independent experiments is shown. *p < 0.05, **p < 0.01, and ***p < 0.001. (C) Invaded cells were stained and quantified by measuring absorbance of the dye at 570 nm. Mean ± SD of absorbance obtained from three independent experiments is shown. (D) Relative wound area of negative control or TROP2 siRNA-transfected and scratched cells in the absence or presence of mitomycin C (MMC, 5 μg/mL). Experiments were performed in six wells for each condition and independently repeated three times. **p < 0.01 and ***p < 0.001.
