Fig. 4

The impact of including a probe enrichment step was assessed using dilutions of O1 Manisa virus in epithelial suspension. In addition, one set of samples was heated to mimic poor quality samples. The inclusion of an enrichment step (green) increased the mapping rate across the genome relative ‘standard’ NGS (orange) for both control/unheated (A) as well as heated/poor quality (B) samples. Enhanced rates of mapping were observed throughout the genome (C), although the impact was particularly conspicuous for reads mapping to the capsid region (shaded grey).