Fig. 1 | Scientific Reports

Fig. 1

From: Development of the membrane ceiling method for in vitro spermatogenesis

Fig. 1

Device design. (A) Schematic illustrations and pictures of the conventional method using agarose gel and PDMS ceiling chip (AG-PC) (left) and our novel method consisting of the membrane ceiling (MC) chip and an oxygen-permeable material (MC method) (right). (B) Picture of the MC method. Scale bar = 25 mm. (C) Picture and schematic illustrations of the MC chip. (D) Representative images of testis tissue in the AG-PC and MC method observed by an inverted microscope (BZ-X700). PDMS was used to line the base of the well plate. Scale bar = 100 μm.

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