Fig. 4
From: Human parietal epithelial cells as Trojan horses in albumin overload
Immunofluorescence microscopy of colocalization of FITC- BSA and megalin or cubilin. Representative immunofluorescent images by confocal microscopy showed protein expression and colocalisation of megalin (A) and cubilin (B) with FITC-BSA. FITC-BSA is shown in green, megalin (A, Alexa Fluor™ 594) and cubilin (B, Alexa Fluor™ 594) in red. In merge images white arrows indicate colocalization as yellow/orange signal. Images are representative of two separate experiments and were analysed by a fluorescence microscope DMI6000CS-TCS SP8 (Leica Microsystem) in confocal mode, with a Z-interval of 1 μm z-interval. Objective: 63X/1.4, oil immersion. Scale bar = 50 μm.
