Fig. 5
From: Human parietal epithelial cells as Trojan horses in albumin overload
Inhibition of BSA-FITC uptake. (A) Effect of receptor-associated protein (RAP) on FITC-BSA uptake in hPECs. hPECs were preincubated with different amounts of RAP for 1 h (white bargraphs) followed by incubation with 1 mg/ml FITC-BSA for 2 h. Black bargraphs display FITC-BSA alone. Data are represented as fluorescence absorbance values normalized to cell number. Results are from two independent experiments performed in triplicate *p < 0.05 (Mann–Whitney U-test). (B) Effect of a sheep polyclonal anti-human Cubilin IgG antibody on FITC-BSA uptake in hPECs. hPECs were preincubated with different amounts of a sheep polyclonal anti-human Cubilin IgG antibody for 3 h (gray bargraphs) followed by incubation with 1 mg/ml FITC-BSA for 2 h. Black bargraphs display FITC-BSA alone. Data are represented as fluorescence absorbance values normalized to cell number. Results are from two independent experiments performed in triplicate *p < 0.05 (Mann–Whitney U-test). (C) Representative confocal images of inhibition by RAP and a sheep polyclonal anti-human Cubilin IgG antibody on FITC-BSA uptake. FITC-BSA was detected as green signal. Images are representative of two separate experiments and were analysed by a fluorescence microscope DMI6000CS-TCS SP8 (Leica Microsystem) in confocal mode, with a Z-interval of 1 μm z-interval. Objective: 63X/1.4, oil immersion. Scale bar = 50 μm.
