Fig. 8
From: Human parietal epithelial cells as Trojan horses in albumin overload
Evaluation of hPECs morphology after albumin overload. (A) Representative microscopy images of hPECs by differential interference contrast (DIC) (grey-scale images) showing cell shape changes at different time and albumin concentration. CTR: untreated cells. Images were acquired using a DMI6000CS-TCS SP8 fluorescence microscope (Leica Microystems) with 40X/0.60 objective. Scale bar 50 μm. (B) Phalloidin fluorescence labelling of F-actin in hPECs after albumin overload for 72 h. Images demonstrating the pattern of F-actin filaments distributed as bundles of stress fibers along the cell axis, arranged neatly and unbranched in untreated cells (CTR). Boxed photos showing zoomed-in area. Stress fibers of the actin cytoskeleton were disrupted: a marked redistribution of F-actin fibers toward the periphery was observed (10 mg/ml), markedly and completely unwrapped at the higher concentration (30 mg/ml). Red: actin; Blue: DAPI. Fluorescence microscope images are representative of three separate experiments. Images were acquired using a DMI6000CS-TCS SP8 fluorescence microscope (Leica Microystems) with 20X/0.4 objective. Scale bar 50 μm. Merge: phalloidin/actin with DAPI. Scale bar 50 μm. (C) Quantification of F-actin intensity: data are reported as mean ± SD of the measured gray-scale values of images from each condition and normalized to cell number counted on the same measured cell area. Results are from three independent experiments **p < 0.005, F = 20.75, using ANOVA and by Bonferroni’s correction.
