Fig. 3 | Scientific Reports

Fig. 3

From: Inosine monophosphate dehydrogenase 2 (IMPDH2) modulates response to therapy and chemo-resistance in triple negative breast cancer

Fig. 3

Doxorubicin resistance is accompanied by increased IMPDH2 activity and enhanced sensitivity to IMPDH2 inhibition. IMPDH activity assay in naïve and DoxoR MDA-MB-231 (A) or 4T1 (B) cells. Data is average −/+ std of 3 independent experiments. Statistics by unpaired Student’s t-test. (C) Doxorubicin survival curve (n = 3) of MDA-MB-231 DoxoR cells transduced with shIMPDH2 (shI2) or its corresponding silencing control (shCtrl). Immunoblot confirms IMPDH2 depletion; actin is used as loading control. Membranes were cut prior to hybridization with antibodies. (D) Doxorubicin survival curve (n = 4) of 4T1 DoxoR cells transduced with shImpdh2 (shI2) or its corresponding silencing control (shCtrl). Immunoblot confirms Impdh2 depletion; actin is used as loading control. Membranes were cut prior to hybridization with antibodies. (E) Ribavirin (RBV) and MPA IC50 in MDA-MB-231 and 4T1 naïve or doxorubicin-resistant cells (n = 4).

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