Fig. 5
IMPDH2 inhibition reduces the tumor growth and metastatic burden of DoxoR cells in vivo. (A) 1 × 106 MDA-MB-231 DoxoR cells were implanted orthotopically in the mammary fat pad of female NSG mice (n = 9). Treatment with PBS or ribavirin (3 mg/Kg in PBS) by i.p. injections (5 days/week) started when tumor reached 100 mm3 (day 0). Tumors were measured twice/week. Animals were humanely euthanized when a tumor in any group reached the limit set by IACUC protocol. Data is average −/+ SEM. Statistics by 2-way ANOVA test. (B) 1.5 × 105 MDA-MB-231 DoxoR cells were injected in the tail vein of female NSG mice (n = 5). Treatment with PBS or ribavirin (10 mg/Kg in PBS) by i.p. injections (5 days/week) started 4 days post-injection. Animals were humanely euthanized after 3 weeks. Experimental metastases were visualized with India ink stain and quantified manually. Statistics by two-tailed Student’s t-test. (C) 1.5 × 105 MDA-MB-231 DoxoR cells transduced with shIMPDH2 (shI2) or corresponding non-silencing control (shCtrl) were injected in the tail vein of female NSG mice (n = 5). Animals were humanely euthanized after 3 weeks. Experimental metastases were visualized with India ink stain and quantified manually. Statistics by two-tailed Student’s t-test. (D) Representative images of India ink-stained lungs from (C).
