Fig. 9

AKBA protects LPS-induced HPMECs via U-STAT1/BST2. (A, B) The proliferation of HPMECs was verified by EDU assay, (100×magnification), n = 3. (C, D) The proliferation of HPMECs was verified by colony formation assay, (100×magnification), n = 3. (E, F) The tube forming ability of HPMECs was evaluated by quantifying the number of nodes and the vascular coverage area, (100×magnification), n = 3. (G, H) The migration function of HPMECs was verified by wound healing assay. (100×magnification), n = 3. (I, J) The migration function of HPMECs was verified by transwell assay. (100×magnification), n = 3. (K) Western blot analysis of BST2, U-STAT1, YP-STAT1, Bad, using β-actin expression as the internal reference in HPMECs, n = 3. (L) Statistical analysis of Western blot of BST2 in HPMECs, n = 3. (M) Statistical analysis of Western blot of U-STAT1 in HPMECs, n = 3. (N) Statistical analysis of Western blot of Bad in HPMECs, n = 3. ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001.