Fig. 1

The relative mRNA and protein levels of LDLR. GAPDH or α-actin served as a loading control. (A) Protein image of the gastrocnemius LDLR, (B) Protein quantitative results of gastrocnemius LDLR, (C) mRNA expression level of LDLR in the gastrocnemius, (D) mRNA expression level of LDLR in the ileum. Data are reported as mean ± SEM. Two-tailed Student’s t-test or Mann–Whitney U test was used for the comparisons between two groups, and one-way analysis of variance (ANOVA) was used for the comparisons among multiple groups. *p < 0.05 vs. Acon group, ǂp < 0.05 vs. ALKd group, ^†p < 0.05 vs. Ocon group. Acon adult mice with empty vector, ALKd adult mice with LDLR knockdown, Ocon old mice with empty vector, OLKd old mice with LDLR knockdown (Each group ≥ 3).