Fig. 4
To evaluate the impact of PD-1/PD-L1 signaling on DLBCL cells in the context of EBV infection. (A) The representative bright-field images were taken from control, 5 µM BMS202, 10 µM BMS202 and 20 µM BMS202 groups at incubation times of 0 and 24 h, respectively. (B–C). Bar chart showing the comparison of CCK8 values of DB or Farage cells incubated with/without BMS202. (D) The relative mRNA expression of PD-L1 and PD in DB or Farage cells from the aforementioned three groups (n = 3 per group). (E) The propidium iodide (PI) flow cytometric assay was implemented in 24-h incubated DB or Farage cells from control and 10 µM BMS202 group. (F) The bar chart showing the comparison of the values of late apoptosis (the upper right corner of the quadrant) + early apoptosis (the lower right corner of the quadrant) in the cultured DB or Farage cells among control and 10 µM BMS202 group. (G) Bioinformatics analysis depicting genes involved in this study, which were mapped using the STRING database to construct the protein–protein interaction network. (H) The relative mRNA expression of TLR4 in DB or Farage cells from the aforementioned three groups (n = 3 per group). Scale bars = 100 μm in A.
