Fig. 5 | Scientific Reports

Fig. 5

From: Transcriptomic analysis of CDL-gated photoperiodic flowering mechanisms in cannabis and their responsiveness to R: FR ratios in controlled environment agriculture

Fig. 5

qPCR data describing the fold change in gene expression in cannabis leaves across SD transition under high and low R:FR conditions. 4 Biological replicates per assay. UB used as reference, delta-delta Ct method used to calculate relative expression change. In each assay, the last long day is set as control (1) except SOC1, in which case Day 7 is the control. Although no change was observed from the treatment by day 3, by the seventh short day, three genes in the study were affected by high RFR ratios: WNK9 (Panel A), COL5 (Panel B), and FT (Panel C). Expression of WNK9 and COL5 both increased significantly. FT expression was significantly reduced, but this downregulation had no impact on downstream SOC1 (Panel D). Student’s t-test was utilized for calculating statistical significance of means separation. One star (*) indicates a P-value below 0.05, while two stars (**) indicates a P-value below 0.005.

Back to article page