Fig. 2
From: Comparison of antibody-scTRAIL Fc fusion proteins with varying valency for EGFR and TRAIL receptors
Binding of antibody-scTRAIL fusion proteins to immobilized DR5 and EGFR analyzed by ELISA. (A) Hexavalent TRAIL molecules on DR5. (B) Trivalent TRAIL molecules on DR5. (C) Bivalent targeted molecules to EGFR. (D) Monovalent targeted molecules to EGFR. scTRAIL-antibody fusion proteins were titrated at a 1:4 dilution between concentrations starting at 100 nM, coated with DR5- or EGFR-moFc (0.3 µg/well), detected with HRP-conjugated anti-human Fc antibody. n = 3, mean ± SD.
