Table 1 Binding EC50 values (ELISA and flow cytometry) and cytotoxic effects of the scTRAIL-antibody fusion proteins to their target antigens DR5 and EGFR and EGFR-positive cell lines Colo205 and HCT116. n = 3; mean ± sd; -: not performed.
From: Comparison of antibody-scTRAIL Fc fusion proteins with varying valency for EGFR and TRAIL receptors
Moieties | Mole- cule | Format | ELISA | Cell binding | Cytotoxicity | |||||
|---|---|---|---|---|---|---|---|---|---|---|
DR5 | EGFR | Colo205 | HCT116 | Colo205 | HCT116 | |||||
Fab/scFv + scTRAIL | EC50 [pM] | EC50 [pM] | EC50 [pM] | EC50 [pM] | EC50 [pM] | Maximal killing [%] | EC50 [pM] | Maximal killing [%] | ||
0 + 2 | #1 | Fc-scTRAIL | 493 ± 53 | - | 5,173 ± 3,085 | 3,850 ± 990 | 36 ± 4 | 100 | 71 ± 30 | 75 |
0 + 2 | #7 | scTRAIL-Fc | 383 ± 64 | - | 4,411 ± 1,725 | 6,177 ± 1,027 | 52 ± 4 | 100 | 177 ± 24 | 75 |
2 + 2 | #3 | IgG-scTRAIL | 655 ± 145 | 291 ± 58 | 86 ± 20 | 129 ± 25.8 | 6 ± 1 | 100 | 6 ± 2 | 75 |
2 + 2 | #4 | scFv-Fc-scTRAIL | 434 ± 22 | 496 ± 232 | 244 ± 141 | 351 ± 46 | 6 ± 1 | 100 | 8 ± 2 | 75 |
2 + 2 | #9 | scTRAIL-Fc-scFv | 1,069 ± 124 | 768 ± 142 | 997 ± 349 | 633 ± 134 | 9 ± 2 | 100 | 12 ± 6 | 75 |
2 + 1 | #5 | IgG-scTRAIL | 1,268 ± 456 | 211 ± 34 | 51 ± 20 | 352 ± 274 | 1,756 ± 1076 | 25 | 256 ± 142 | 50 |
2 + 1 | #6 | scFv-Fc-scTRAIL | 753 ± 71 | 2,344 ± 16 | 73 ± 22 | 188 ± 147 | 1,536 ± 1155 | 38 | 499 ± 276 | 55 |
1 + 1 | #11 | scFv-scTRAIL-Fc | 883 ± 381 | 767 ± 28 | 258 ± 123 | 1,492 ± 999 | 418 ± 211 | 50 | 472 ± 60 | 60 |
1 + 1 | #10 | Fab-scTRAIL-Fc | 929 ± 185 | 1,641 ± 144 | 255 ± 23 | 746 ± 65 | 1,385 ± 449 | 50 | 727 ± 43 | 60 |
0 + 1 | #2 | DIgG-Fc-scTRAIL | 1,283 ± 295 | - | 3,153 | 72,000 | 601 ± 75 | 100 | 844 ± 445 | 70 |
0 + 1 | #8 | DFab-scTRAIL-Fc | 853 ± 208 | - | 5,680 | 18,000 | 139 ± 42 | 100 | 264 ± 141 | 70 |
