Table 2 Validation parameters of Jasem steroid hormones LC-MS/MS analysis kit.
From: Comparative analysis of neurosteroid levels in normal and adenomatous human pituitary tissue
Analytes | LOD (µg/L) | LOQ (µg/L) | Linearity (R2) | Accuracy (%) | Repeatability (RSD%) |
|---|---|---|---|---|---|
Pregnenolone | 0.033 | 0.108 | 0.994 | 77.49 | 10.46 |
7α-Hydroxypregnenolone | 0.012 | 0.038 | 0.998 | 98.54 | 3.8 |
17-Hydroxypregnenolone | 0.145 | 0.480 | 0.997 | 100.44 | 9.24 |
Dehydroepiandrosterone | 0.102 | 0.336 | 0.998 | 109.62 | 6.66 |
Dehydroepiandrosterone Sulfate | 4.945 | 16.32 | 0.997 | 103.47 | 2.63 |
4-Androstene 3,17-dione | 0.023 | 0.076 | 0.998 | 109.93 | 1.99 |
Testosterone | 0.008 | 0.026 | 0.997 | 96.75 | 1.8 |
Dihydrotestosterone | 0.012 | 0.041 | 0.997 | 101.98 | 14.36 |
Estradiol (Oestradiol) | 0.015 | 0.051 | 0.998 | 109.37 | 6.32 |
Androsterone | 0.035 | 0.115 | 0.994 | 93.7 | 13 |
Progesterone | 0.033 | 0.108 | 0.994 | 108.37 | 4.45 |
Allopregnanolone | 0.011 | 0.035 | 0.998 | 97.45 | 4.6 |
11-Deoxycorticosterone | 0.016 | 0.052 | 0.994 | 95.34 | 6.84 |
Corticosterone | 0.054 | 0.179 | 0.998 | 98.42 | 4.09 |
Aldosterone | 0.011 | 0.035 | 0.998 | 99.81 | 6.34 |
17-Hydroxyprogesterone | 0.028 | 0.091 | 0.997 | 97.01 | 4.8 |
11-Deoxycortisol | 0.015 | 0.048 | 0.998 | 102.23 | 4.45 |
Cortisol | 0.102 | 0.336 | 0.999 | 89.74 | 1.69 |
