Fig. 3
Quantification of all tumor cells/clusters along the invasive front in a large series of colorectal cancer (CRC) cases. (A) Pie chart depicting percentages of tumor cells/clusters of a particular size out of all tumor cells/clusters ≤ 10 cells automatically detected across the entire invasive margin of all 1134 pTNM stage I-III CRC cases. (B) Pie chart depicting percentages of tumor cells/clusters of a particular size out of all tumor cells/clusters ≤ 4 cells detected and considered tumor budding (TB). (C) Pie chart depicting percentages of tumor cells/clusters of a particular size out of all tumor clusters detected with a size between 5 and 10 cells and considered poorly differentiated clusters (PDCs). (D) Percentage overlap of TB and PDC hotspots in all cases as well as the hotspots of increasing cluster size cut-off through the tiered scoring. (E) Table depicting the statistics of tiered scoring with cut-offs of only single cells and inclusion of cell clusters of incrementally increasing size. The mean number of tumor cells/clusters within a hotspot of each grouping, the distribution (%) of cases between low and high-grade groups, and hazard ratios for each grouping are depicted.
