Fig. 4
FOXO3 binds to the ATG4A promoter and regulates ATG4A expression. (A) Bioinformatic algorithms predicted that ATG4A is a target gene of the transcription factor FOXO3 and that there are several possible binding sites in the ATG4A promoter region. (B) After overexpressing FOXO3 in LNCaP and PC-3 cells, the protein and mRNA expression of ATG4A was measured by western blot and qRT-PCR, respectively. (C) 293T cells were co-transfected with the luciferase reporter vector containing the ATG4A promoter and the FOXO3 overexpression vector. Then, the luciferase density was measured. (D) 293T cells were co-transfected with the luciferase reporter vector containing the truncated ATG4A promoter and the FOXO3 overexpression vector. Then, the luciferase density was measured. (E) Luciferase reporter vector containing the ATG4A promoter sequence, the wild type (WT) or the corresponding mutant (MUT), was co-transfected with the FOXO3 overexpression vector in 293T cells. Then, the luciferase density was measured.
