Fig. 6 | Scientific Reports

Fig. 6

From: Sinularin induces autophagy-dependent cell death by activating ULK1 and enhancing FOXO3-ATG4A axis in prostate cancer cells

Fig. 6

Sinularin induces prostate cancer cell autophagy through ATG4A. (A) Western blot analysis was used to determine ATG4A protein expression after ATG4A was knocked down by siRNAs. (B) Representative immunofluorescence images and mean fluorescence intensity (MFI) quantification of LC3 taken from PC-3 cells treated with Sinularin or subjected to ATG4A knockdown. Autophagosomes were visualized and examined via immunofluorescence microscopy through photographs of LC3 puncta. (C) Western blot analysis to determine the LC3 I/II and p62 levels in Sinularin-treated LNCaP and PC-3 cells following ATG4A knockdown.

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