Fig. 1
From: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei
Impact of TbCDA-like modulation on growth and protein expression in T. brucei. (a) Growth curves of Tb PF and Tb PF CDA-like-RNAi cell lines. DOX, doxycycline. Each point represents the mean from three biological replicates. (b) Western blot quantification demonstrating efficient reduction of TbCDA-like levels following DOX induction. (c) Growth curves of Tb PF and the Tb PF CDA-like-OE cell lines with or without DOX. Data point represents the mean from three biological replicates. (d) Western blot quantification of TbCDA-like expression showing the increase in TbCDA-like expression upon DOX induction. 5 × 106 parasites were loaded in each lane. Anti-TbCDA-like (1:2500) and anti-β-tubulin (1:5000) antibodies were used for detection and normalization, respectively. Protein quantification was performed using Fiji software. Samples and controls derived from the same experiment and blots were processed in parallel. Only the lines of interest are shown from full-length blots. Original blots are presented in Supplementary Fig. S4.
