Fig. 3
From: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei
Altered cell cycle progression in T. brucei upon TbCDA-like overexpression. (a) Distribution of cells across different cell cycle phases after 2 days of overexpression induction, followed by propidium iodide staining and flow cytometry (FACS) analysis. Data are presented as mean ± SD from 3 independent experiments. (b) Quantification of nuclei (N) and kinetoplasts (K) after 2 days of overexpression induction, followed by DAPI staining and fluorescence microscopy. N* and K* denote elongated nuclei and kinetoplasts, respectively, indicative of mitosis or kinetoplast division. Data are presented as mean ± SD from approximately 300 cells from 3 independent experiments. Asterisks indicate significant differences determined by ANOVA. *p < 0.05; ***p < 0.001. The statistical significance analyse was performed by comparing the OE + DOX cell line with the Tb PF cell line. (c) Representative fluorescence microscopy images (Zeiss Axio Imager A1) after DAPI staining, illustrating the different cell populations.
