Fig. 4
From: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei
Modulation of TbCDA-like expression induces DNA damage and alters the distribution of nuclear foci in T. brucei cell lines. (a) Percentage of γH2A-positive cells in Tb PF, Tb CDA-like-RNAi and Tb CDA-like-OE cell lines after 2 days of induction. Data are presented as mean ± SD of approximately 250 cells from 3 independent experiments. (b) Distribution of cells displaying 1 focus, > 1 focus (2–4 foci) or whole nucleus labeling for γH2A after 2 days of induction. (c) Representative immunofluorescence images showing nuclei labeled for γH2A, with 1 focus, > 1 focus or the whole nucleus. Images were captured using a Zeiss Axio Imager A1 fluorescence microscope following DAPI staining and immunolabeling with a polyclonal anti-γH2A antibody, followed by an anti-rabbit IgG conjugated to Alexa Fluor 488. Asterisks indicate significant differences determined by ANOVA. ***p < 0.001. The statistical significance analysis was performed by comparing the OE + DOX cell line with the Tb PF cell line.
