Fig. 5
From: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei
TbCDA-like is localized in the cytosol. Localization of TbCDA-like fused to 6x c-myc at the N-terminus in Tb PF N-ter KI cells and of TbCDA-like fused to 12x c-myc at the C-terminus in the Tb PF C-ter KI cell line. The parental Tb PF line was used as a negative control. Immunolabeling was performed with a mouse monoclonal anti-c-myc antibody and Alexa Fluor 488-conjugated anti-mouse IgG. Mitochondria were stained with MitoTracker Red CMXRos, and the nucleus and kinetoplast were visualized using DAPI. Images were captured using an Olympus IX81 microscope and deconvoluted using the Huygens Essential software (version 3.3; Scientific Volume Imaging). Images were analyzed with Fiji/ImageJ software. Bar, 10 μm.
