Fig. 6
From: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei
TbCDA-like relocalizes to perinuclear granules under starvation conditions, with an increase in number and a decrease in size compared to untreated cells. (a) Immunofluorescence images showing the distribution of TbCDA-like in the TbCDA-like KI line with 6x c-myc fusion at the N-terminus (Tb PF N-ter KI) under normal (0 min control) and starvation conditions for 60, 120, or 180 min. Cells were stained with anti-c-myc monoclonal antibody and Alexa Fluor 488-conjugated anti-mouse IgG secondary antibody. Nuclei and kinetoplasts were labeled with DAPI. Scale bar, 10 μm. (b) 3D construction of images obtained with the three-dimensional reconstruction module of Leica Application Suite X software. (c) Quantification of the number of granules per cell, with a minimum granule area of 0.05 μm2. Data represent the mean of at least 20 cells per condition, with boxes showing the interquartile range and whiskers indicating the maximum and minimum values. (d) Analysis of the granule area per cell, with a minimum area of 0.05 μm2. Data are presented as the mean ± SD for 20–22 cells per condition. Statistical significance was determined by ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001). Images were analyzed using Fiji/ImageJ software.
