Fig. 7
From: A cytidine deaminase-like protein modulates pyrimidine nucleotide homeostasis in Trypanosoma brucei
TbCDA-like colocalizes with stress granule marker proteins in T. brucei. (a) Colocalization of TbCDA-like with stress granules marker proteins TbDhh1, TbDRBD3 and TbScd6 under normal (control) and starvation conditions (120 min) in the TbCDA-like KI line with 6x c-myc fusion at the N-terminus. An anti-c-myc monoclonal antibody and the corresponding anti-Dhh1, anti-DRBD3 or anti-Scd6 polyclonal antibodies were used as primary antibodies. Alexa Fluor 488-conjugated anti-mouse IgG and Alexa Fluor 594-conjugated anti-rabbit IgG were used as secondary antibodies. Nuclei and kinetoplasts were labelled with DAPI. Images were acquired using a Leica DMi8 microscope and deconvoluted with Huygens Essential software (version 3.3; Scientific Volume Imaging). Bar, 10 μm. (b) Quantification of granules per cell with a minimum area of 0.05 μm2. Data represent the mean from at least 10 cells per condition, with boxes indicating the interquartile range and whiskers showing the maximum and minimum values. (c) Granule area per cell analysis, considering a minimum granule area of 0.05 μm2. Data are presented as mean ± SD from 10–22 cells per condition. Statistical significance was determined by ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001). Images were analyzed using Fiji/ImageJ software.
