Fig. 4 | Scientific Reports

Fig. 4

From: ANXA2 regulates mitochondrial function and cellular senescence of PDLCs via AKT/eNOS signaling pathway under high glucose conditions

Fig. 4

The effect of high glucose and ANXA2 on Mitophagy of PDLCs. PDLCs were transfected with pcDNA3.1-ANXA2 or its empty vector controls, followed by treatment with high glucose or CQ (chloroquine, 10µM) (A) The expression of LC3, p62, Parkin at protein level in PDLCs. (B–D) Semi-quantitative analysis of LC3-II/I, p62, Parkin. (E) The expression of LC3 protein level in PDLCs with CQ. (F) Semi-quantitative analysis of LC3-II/I. All data are presented as the means ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. CTRL: Control; 40G: 40mM glucose; 40G-A: 40mM glucose + ANXA2 overexpression. 40G-A-CQ: 40mM glucose + ANXA2 overexpression + chloroquine.

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