Fig. 5 | Scientific Reports

Fig. 5

From: ANXA2 regulates mitochondrial function and cellular senescence of PDLCs via AKT/eNOS signaling pathway under high glucose conditions

Fig. 5

The effect of ANXA2 on AKT/eNOS signaling pathway. PDLCs were transfected with siRNA, pcDNA3.1-ANXA2 or its empty vector controls. (A) The expression of Akt, p-Akt (Ser473), eNOS, p-eNOS(Ser1177) at protein level in PDLCs after knockdown of ANXA2. (B–D) Semi-quantitative analysis of ANXA2, p-Akt/Akt, p-eNOS/ eNOS. (E) The expression of Akt, p-Akt (Ser473), eNOS, p-eNOS(Ser1177) at protein level in PDLCs after overexpression of ANXA2. (F–H) Semi-quantitative analysis of ANXA2, p-Akt/Akt, p-eNOS/ eNOS. All data are presented as the means ± SEM from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

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